WebJan 19, 1999 · High spatial frequencies in the illuminating light of microscopes lead to a shift of the object spatial frequencies detectable through the objective lens. If a suitable procedure is found for evaluation of the measured data, a microscopic image with a higher resolution than under flat illumination can be obtained. A simple method for generation … WebAug 25, 2024 · Abstract: We propose a chiral imaging modality based on optical chirality engineering, fluorescence-detected circular dichroism and structured illumination …
[1908.09391] Chiral Structured Illumination Microscopy
WebApr 6, 2024 · Recently, chiral structured illumination microscopy has been proposed to image fluorescent chiral domains at sub-wavelength resolution. Chiral structured illumination microscopy is based on the combination of structured illumination microscopy, fluorescence-detected circular dichroism, and optical chirality engineering. … WebThe aim of this work is to clarify the origins of second‐harmonic generation circular dichroism response in complicated three‐dimensional biological systems and combine the axial resolving power of second-harmonicgeneration and chiral sensitivity to realize three-dimensional chiral detection in biological tissues. Chirality is one of the most … lapan satellite
[1908.09391] Chiral Structured Illumination Microscopy
Web2x increase in spatial resolution over wide-field microscopy. In 2D/3D-SIM, lateral only or both lateral and axial resolutions are improved, with respectively ~100 nm in xy and ~250 nm in z. 4D imaging at fast frame rate. Typically, 9-15 raw images are needed for 2D/3D-SIM, whereas STORM/PALM requires 1000 (or more) raw images and STED, as a ... WebStructured illumination microscopy (SIM) is based on the excitation of the sample with a known spatially structured pattern of light and relies on the generation of interference patterns known as Moiré effect [7]. Different images are acquired, and by mathematically deconvolving the interference signal, a super-resolution image is obtained. WebWe propose a chiral imaging modality based on optical chirality engineering, fluorescence-detected circular dichroism, and structured illumination microscopy. In this method, … assistenza vuse