WebRun CYTO-COMP™ Cells staine d with CYTO-C OMP Reagents or run other compenstation reagents to calibrate your system's color compensation. Record peak positions, high voltages, and color compensation settings in a logbook. Use COULTER Immuno-Trol™ Cells or CYTO-TROL™ Control Cells to verify cell surface marker panels. WebProduct No: 6607023. CYTO-COMP Cell Kit is a preparation of lyophilized human lymphocytes and reconstitution buffer used in conjunction with the CYTO-COMP Reagent Kit to establish and monitor color compensation settings for multicolor …
Mixed-mode Sorting with CytoFLEX SRT Cell Sorter
WebMay 29, 2016 · The present chapter focuses on the validation of the following analytical methods for the control of mesenchymal stromal cells (MSC) for cell therapy clinical trials: Microbiological control for cellular product Endotoxin assay Mycoplasma assay Cell count and viability Immunophenotype Clonogenic potential (CFU-F assay) Web2. Set up control material (e.g. peripheral blood or cyto comp cells) in single tubes stained with single color antibodies for every PMT (recommend antibodies which identify "positive" and "negative" populations (e.g. CD2, CD3, CD4, CD5 etc) 3. Use a protocol containing FS vs SS as well as dotplots with all the PMTs vs SS for this particular ... raymour and flanigan mckinley sofa
Flow Cytometry Support Reagents - Beckman
WebFeb 10, 2024 · Stained Cyto-Comp™ cells were applied to compensate the fluorescence overlap. The flow cytometer fluidic stability and the optical alignment were daily tested using Flow-Check™ Fluorospheres (Beckman Coulter, Krefeld, Germany). For verification, Immunotrol cells (Beckman Coulter) were applied three times a day. WebJan 31, 2024 · To assure data quality, flow rate, laser power calibration, and event dispersion of the FC 500 flow cytometer were checked on a daily basis using Flow‐Check Pro Fluorospheres (Beckman Coulter). Autosetups with Cyto‐Comp Cells (Beckman Coulter) were routinely repeated for the automated compensation matrices. WebFlow-Set Fluorospheres are a suspension of fluorospheres (fluorescent microbeads) used as an aid in optimizing a flow cytometer for quantitative analysis of human leukocytes. Each fluorosphere contains a dye which has a fluorescent emission range of 525 nm to 700 nm when excited at 488 nm. simplify single application lawn fertilizer