WebExpression of p140Cap EPLYA/EGLYA double mutant partially rescues Src activation. cDNAs encoding GFP, GFP-p140Cap WT and GFP-p140Cap EPLYA/EGLYA double mutant were used to transfect HEK-293 cells ... Webmedium (DMEM) (GibcoBRL) supplemented with 10% FBS (Hy-clone), penicillin (100 U/mL; GibcoBRL), and streptomycin sulfate (100 mg/mL; GibcoBRL). pdSMC1A and pdSMC2 cells arose from explant cultures of surgical specimens obtained from 2 patients with grossly diseased atherosclerotic plaque present in an aortic aneurysm and a carotid artery ...
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WebThen, the membranes were hy-bridized with the IRDye secondary antibodies at room temperature for 1.5 h. The immunoreactive bands were ... 10% FBS to each well at 37 C and incubated for an ... WebDec 18, 2024 · b. Seed 2.5 mL of cells from step 2 into each plate (total ∼4 × 10 6 cells/plate). Incubate the plates 12–18 h or until 70%–80% confluence is reached. Note: For concentrated lentiviral vectors, up to six 150 mm plates can be used in a single production run, with capacity being limited by ultracentrifuge space. siby tm rezepte
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Webwithout FBS. These plates were incubated at 37 C in a humidified CO 2 incubator for 90 min, and then each plate was covered with 5 mL of a standard over-lay containing 0.5% agarose and 6% FBS in DMEM without phenol red (the infection medium was not re-moved prior to adding the overlay). The plates were again incubated at 37 C in a humidified ... WebMedium DMEM/FBS HY Dex/Asc/b-GP rhBMP-2 (mg/ml) (+, ) (ng/ml) 000 + 0 0 100 + 1.0 0 200 + 4.0 0 010 + 0 + 0 110 + 1.0 + 0 210 + 4.0 + 0 001 + 0 10 101 + 1.0 10 201 + 4.0 10 011 + 0 + 10 111 + 1.0 + 10 211 + 4.0 + 10 DMEM=Dulbecco’s modified Eagle’s medium; FBS=fetal bovine serum;HY=sodiumhyaluronate;Dex=dexamethasone;Asc=ascor- the perfume shop card